Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene

International Journal of Molecular Sciences

Guosheng Wang¹  Xingbo Zhao³  Juming Zhong²  Meng Cao³  Qinghua He¹  Zhengxin Liu¹  Yaqiu Lin¹  Yaou Xu¹ 

[1] College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China; E-Mails:;College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA; E-Mail:;College of Animal Science, China Agricultural University, Beijing 100193, China; E-Mails:

关键词: Bos grunniens;    lactate dehydrogenase;    polymorphism;    hypoxic adaptation;   

DOI  :  10.3390/ijms140611994

来源: mdpi

【 摘 要 】

The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD⁺ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak.

【 授权许可】

CC BY   
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