Combining Hexanoic Acid Plant Priming with Bacillus thuringiensis Insecticidal Activity against Colorado Potato Beetle

International Journal of Molecular Sciences

Inmaculada Garc໚-Robles³  Camila Ochoa-Campuzano³  Emma Fernández-Crespo²  Gemma Cama༞s²  Amparo C. Martínez-Ramírez³  Carmen González-Bosch¹  Pilar Garc໚-Agustín²  Carolina Rausell³ 

[1] Department of Biochemistry and Molecular Biology, University of Valencia, IATA (CSIC), Paterna, Valencia 46980, Spain; E-Mail:;Biochemistry and Biotechnology Laboratory, Plant Physiology Area, Department CAMN, University Jaume I, Castellón 12071, Spain; E-Mails:;Department of Genetics, University of Valencia, Dr. Moliner 50, Burjassot 46100, Valencia, Spain; E-Mails:

关键词: Colorado potato beetle;    Bacillus thuringiensis;    Cry3Aa toxin;    intestain proteases;    proteolysis;    Solanaceae;    hexanoic acid;    priming;    plant defense;    plant hormones;   

DOI  :  10.3390/ijms140612138

来源: mdpi

【 摘 要 】

Interaction between insect herbivores and host plants can be modulated by endogenous and exogenous compounds present in the source of food and might be successfully exploited in Colorado potato beetle (CPB) pest management. Feeding tests with CPB larvae reared on three solanaceous plants (potato, eggplant and tomato) resulted in variable larval growth rates and differential susceptibility to Bacillus thuringiensis Cry3Aa toxin as a function of the host plant. An inverse correlation with toxicity was observed in Cry3Aa proteolytic patterns generated by CPB midgut brush-border membrane vesicles (BBMV) from Solanaceae-fed larvae, being the toxin most extensively proteolyzed on potato, followed by eggplant and tomato. We found that CPB cysteine proteases intestains may interact with Cry3Aa toxin and, in CPB BBMV from larvae fed all three Solanaceae, the toxin was able to compete for the hydrolysis of a papain substrate. In response to treatment with the JA-dependent plant inducer Hexanoic acid (Hx), we showed that eggplant reduced OPDA basal levels and both, potato and eggplant induced JA-Ile. CPB larvae feeding on Hx-induced plants exhibited enhanced Cry3Aa toxicity, which correlated with altered papain activity. Results indicated host-mediated effects on B. thuringiensis efficacy against CPB that can be enhanced in combination with Hx plant induction.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland