International Journal of Molecular Sciences | |
Significant Decline in Galactomannan Signal during Storage of Clinical Serum Samples | |
Gemma L. Johnson1  Shah-Jalal Sarker3  Kate Hill4  Dimitris A. Tsitsikas2  Amelie Morin2  Stephen A. Bustin5  | |
[1] Blizard Institute of Cell and Molecular Science, Queen Mary University of London, London E1 2AT, UK; E-Mail:;Department of Haemato-Oncology, St Bartholomew’s Hospital, London EC1A 7BE, UK; E-Mails:;Centre for Experimental Cancer Medicine, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK; E-Mail:;Division of Infection, Barts Health NHS Trust, London E1 2ES, UK; E-Mail:;Postgraduate Medical Institute, Faculty of Health, Social Care & Education, Anglia Ruskin University, Chelmsford CM1 1SQ, UK; E-Mail: | |
关键词: invasive aspergillosis; platelia; galactomannan; | |
DOI : 10.3390/ijms140712970 | |
来源: mdpi | |
【 摘 要 】
Galactomannan (GM) is widely used for detection of invasive aspergillosis in high-risk haemato-oncology patients. Recent publications have reported a lack of repeatability of GM detection. The objective of this retrospective study was to assess the repeatability of GM levels during storage of clinical samples. In a GM screening strategy, positive sera were repeat tested as per manufacturer’s recommendations. Short-term (ST) storage of samples was at +4 °C while long-term (LT) storage was at −80 °C. Bronchoalveolar (BAL) fluid was also repeating tested after ST storage and LT storage. Wilcoxon Signed Ranks Test was employed to assess the repeatability of GM levels. In a subset of 14 GM positive sera, repeat testing was performed on both the original serum and ethylenediaminetetraacetic acid (EDTA) pre-treated sample. There was a significant reduction in GM signals on repeat testing following ST storage (median GM index: 0.65
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland
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