Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity

Molecules

Youwu Huang¹  Liang Chen¹  Li Feng¹  Fujiang Guo² 

[1] id="af1-molecules-18-07549">School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, Chi

关键词: Spatholobus suberectus;    phenolic components;    HPLC-DAD/ESI-MS;    procyanidins;    human neutrophil elastase;   

DOI  :  10.3390/molecules18077549

来源: mdpi

【 摘 要 】

Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC₅₀ was found to be 1.33 μg/mL.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.