Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor

Sensors

Hua Kuang¹  Liqiang Liu¹  Liguang Xu¹  Wei Ma¹  Lingling Guo¹  Libing Wang² 

[1]State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China

[2] E-Mails:

[3]Research Centre of Hunan Entry–Exit Inspection and Quarantine Bureau, Changsha 410001, China

[4] E-Mail:

关键词: di-n-butyl phthalate;    ELISA;    liquor;    monoclonal antibody;   

DOI  :  10.3390/s130708331

来源: mdpi

【 摘 要 】

A monoclonal antibody specifically recognizing dibutyl phthalate (DBP) was prepared based on a hapten (di-n-butyl-4-aminophthalate). After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 50% inhibition concentration value (IC₅₀) 33.6 ± 2.5 ng/mL. A low level of cross-reactivity (<5%) was found for other phthalate esters. Recovery tests were conducted using liquor simulant (a mixture of water and ethanol) at two fortification levels (100 ng/mL and 300 ng/mL). The recovery rates ranged from 84.7% to 94.5% with a coefficient of variation between 7.1% and 12.8%. Nine liquor samples of different alcoholic strengths were detected using the proposed measure and confirmatory analysis was performed using liquid chromatography-mass spectroscopy (LC-MS). The detection results showed good consistency between the two measures and all the data above indicated that the proposed ELISA could be applied in DBP screening.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.