Hepatotoxicity Evaluation of Aqueous Extract from Scutia buxifolia

Molecules

Robson Borba de Freitas⁵  Bruno Tomazele Rovani⁵  Aline Augusti Boligon⁵  Thiele Faccim de Brum⁵  Mariana Piana⁵  Roberta da Silva Jesus⁵  Carolina Fantinel Veloso¹  Helena Kober²  Rafael Noal Moresco³  Araldi Isabel Cristina da Costa⁴  Bauermann Liliane de Freitas⁴ 

[1] Graduate Program in Human Communication Disorders, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil;Graduate Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil;Department of Clinical and Toxicological Analysis, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil;Department of Physiology and Farmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil;Graduate Program in Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil

关键词: Scutia buxifolia;    hepatotoxicity;    transaminases;    redox status;    HPLC/DAD;   

DOI  :  10.3390/molecules18077570

来源: mdpi

【 摘 要 】

Nowadays there is an increase in the number of people taking herbals worldwide. Scutia buxifolia is used for the treatment of hypertension, but little is known about its action on liver. Thirty-two Wistar rats were divided into four groups: control and groups treated during 30 days with 100, 200 and 400 mg of lyophilized aqueous extract of S. buxifolia (SBSB)/kg of body weight. This study was planned to explore hepatotoxic effect of SBSB, which was assessed by serum transaminases (ALT and AST). Thiobarbituric acid reactive substances (TBARS) levels were determined in liver, along with thiols content (NPSH), catalase (CAT) activity and, superoxide dismutase (SOD) enzymes. Histopathological studies of liver tissue were performed. Flavonoids and phenolics were quantified in SBSB by high performance liquid chromatography with diode array detection (HPLC/DAD). We did not observe alterations on redox status (TBARS, NPSH, CAT and, SOD) in the control and experimental groups. An increase on AST activity was only observed at 200 mg of SBSB, whereas ALT score was not affected by SBSB. Moreover, no morphological alterations were observed on the hepatocytes, matching the analysed biochemical parameters. This way, we conclude that SBSB was not toxic.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.