Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability

Molecules

Wanling Wu²  Dongye Li²  Yu Zong²  Hong Zhu¹  Defeng Pan¹  Tongda Xu¹  Tao Wang² 

[1] Department of Cardiology, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China;Institute of Cardiovascular Disease Research, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China

关键词: luteolin;    anti-inflammatory;    bone marrow macrophages;    mRNA stability;   

DOI  :  10.3390/molecules18078083

来源: mdpi

【 摘 要 】

Luteolin (Lut) is a common dietary flavonoid present in Chinese herbal medicines that has been reported to have important anti-inflammatory properties. The purposes of this study were to observe the inhibition of lipopolysaccharide (LPS)-induced inflammatory responses in bone marrow macrophages (BMM) by Lut, and to examine whether this inhibition involves p38/MK2/TTP-mediated mRNA stability. Lut suppressed the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in a dose-dependent manner according to enzyme-linked immunosorbent assay (ELISA) analysis. Lut also shortened the half-lives of the TNF-α and IL-6 mRNAs according to real-time PCR analysis. Western blots were performed to assess the activation of p38 and MK2 as well as the expression of TTP. The results indicated that Lut inhibited p38 and MK2 phosphorylation while promoting TTP expression. These results suggest that the anti-inflammatory effects of Lut are partially mediated through p38/MK2/TTP-regulated mRNA stability.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.