Anti-Inflammatory Activity of Different Agave Plants and the Compound Cantalasaponin-1

Molecules

Nayeli Monterrosas-Brisson¹  Martha L. Arenas Ocampo²  Enrique Jiménez-Ferrer¹  Antonio R. Jiménez-Aparicio²  Alejandro Zamilpa¹  Manases Gonzalez-Cortazar¹  Jaime Tortoriello¹ 

[1] Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, Mexico; E-Mails:;Doctorado en Desarrollo de Productos Bióticos, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P. O. Box 24, Yautepec 62730, Morelos, Mexico; E-Mails:

关键词: Agavaceae;    A. angustifolia Haw;    A. tequilana Weber;    A. americana;    cantalasaponin-1;    inflammation;   

DOI  :  10.3390/molecules18078136

来源: mdpi

【 摘 要 】

Species of the agave genus, such as Agave tequilana, Agave angustifolia and Agave americana are used in Mexican traditional medicine to treat inflammation-associated conditions. These plants’ leaves contain saponin compounds which show anti-inflammatory properties in different models. The goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema induced in mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) technique. A dose of 6 mg/ear of acetone extract from the three agave species induced anti-inflammatory effects, however, the one from A. americana proved to be the most active. Different fractions of this species showed biological activity. Finally the F5 fraction at 2.0 mg/ear induced an inhibition of 85.6%. We identified one compound in this fraction as (25R)-5α-spirostan-3β,6α,23α-triol-3,6-di-O-β-D-glucopyranoside (cantalasaponin-1) through ¹H- and ¹³C-NMR spectral analysis and two dimensional experiments like DEPT NMR, COSY, HSQC and HMBC. This steroidal glycoside showed a dose dependent effect of up to 90% of ear edema inhibition at the highest dose of 1.5 mg/ear.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.