International Journal of Molecular Sciences | |
Cultivation of Keratinocytes and Fibroblasts in a Three-Dimensional Bovine Collagen-Elastin Matrix (Matriderm®) and Application for Full Thickness Wound Coverage |
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Jasper Killat1  Kerstin Reimers1  Claudia Y. Choi1  Sabrina Jahn1  Peter M. Vogt1  | |
[1] Department of Plastic, Hand- and Reconstructive Surgery, Hannover Medical School, Hannover D-30659, Germany; | |
关键词: skin substitute; elastin-collagen bovine matrix; Matriderm®; epidermal stem cells; fibroblasts; keratinocytes; | |
DOI : 10.3390/ijms140714460 | |
来源: mdpi | |
【 摘 要 】
New skin substitutes for burn medicine or reconstructive surgery pose an important issue in plastic surgery. Matriderm® is a clinically approved three-dimensional bovine collagen-elastin matrix which is already used as a dermal substitute of full thickness burn wounds. The drawback of an avital matrix is the limited integration in full thickness skin defects, depending on the defect size. To further optimize this process, Matriderm® has also been studied as a matrix for tissue engineering of skin albeit long-term cultivation of the matrix with cells has been difficult. Cells have generally been seeded onto the matrix with high cell loss and minimal time-consuming migration. Here we developed a cell seeded skin equivalent after microtransfer of cells directly into the matrix. First, cells were cultured, and microinjected into Matriderm®. Then, cell viability in the matrix was determined by histology
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland
【 预 览 】
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RO202003190034718ZK.pdf | 5942KB | download |