Production, Characterization, and Antioxidant Activity of Fucoxanthin from the Marine Diatom Odontella aurita

Marine Drugs

Song Xia²  Ke Wang²  Linglin Wan²  Aifen Li²  Qiang Hu¹ 

[1] Laboratory for Algae Research and Biotechnology, College of Technology and Innovation, Arizona State University, 7001 E. Williams Field Road, Mesa, AZ 85212, USA;Institute of Hydrobiology, Jinan University, Guangzhou 510632, China; E-Mails:

关键词: microalgae;    Odontella aurita;    fucoxanthin;    photobioreactor;    antioxidant;   

DOI  :  10.3390/md11072667

来源: mdpi

【 摘 要 】

The production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L⁻¹ and maximum fucoxanthin concentration of 18.47 mg g⁻¹ were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter), resulting in a fucoxanthin volumetric productivity of 7.96 mg L⁻¹ day⁻¹. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC₅₀) of 1,1-dihpenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical being 0.14 and 0.03 mg mL⁻¹, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.