Aqueous Microwave-Assisted Solid-Phase Synthesis Using Boc-Amino Acid Nanoparticles

Applied Sciences

Keiko Hojo¹  Natsuki Shinozaki²  Yoshimi Nozawa²  Yoshinobu Fukumori² 

[1] Faculty of Pharmaceutical Sciences & Cooperative Research Center of Life Sciences, Kobe Gakuin University, Chuo-ku, Kobe 650-8586, Japan;

关键词: aqueous synthesis;    solid-phase peptide synthesis;    microwave assisted synthesis;    nanoparticles;    Boc chemistry;   

DOI  :  10.3390/app3030614

来源: mdpi

【 摘 要 】

We have previously developed water-based microwave (MW)-assisted peptide synthesis using Fmoc-amino acid nanopaticles. It is an organic solvent-free, environmentally friendly method for peptide synthesis. Here we describe water-based MW-assisted solid-phase synthesis using Boc-amino acid nanoparticles. The microwave irradiation allowed rapid solid-phase reaction of nanoparticle reactants on the resin in water. We also demonstrated the syntheses of Leu-enkephalin, Tyr-Gly-Gly-Phe-Leu-OH, and difficult sequence model peptide, Val-Ala-Val-Ala-Gly-OH, using our water-based MW-assisted protocol with Boc-amino acid nanoparticles.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.