Isoliquiritigen Enhances the Antitumour Activity and Decreases the Genotoxic Effect of Cyclophosphamide

Molecules

Hong Zhao²  Xuan Yuan³  Defang Li²  Hongmei Chen²  Jiangtao Jiang²  Zhiping Wang³  Xiling Sun¹ 

[1] School of integrated traditional Chinese and Western Medicine, Binzhou Medical College, Yantai 264000, China;School of Pharmacy, Shihezi University, Shihezi 832002, China;Lanzhou University Second Hospital, Lanzhou University, Lanzhou 730000, China

关键词: isoliquiritigenin;    antitumour;    cyclophosphamide;    genotoxic effect;   

DOI  :  10.3390/molecules18088786

来源: mdpi

【 摘 要 】

The aim of this study was to evaluate the antitumour activities and genotoxic effects of isoliquiritigenin (ISL) combined with cyclophosphamide (CP) in vitro and in vivo. U14 cells were treated with either of ISL (5–25 μg/mL) or CP (0.25–1.25 mg/mL) alone or with combination of ISL (5–25 μg/mL) and CP (1.0 mg/mL) for 48 h. The proliferation inhibitory effect in vitro was evaluated by MTT and colony formation assays. KM mice bearing U14 mouse cervical cancer cells were used to estimate the antitumour activity in vivo. The genotoxic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronuclei. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results showed that ISL enhanced antitumour activity of CP in vitro and in vivo, and decreased the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.