Polymorphisms of the Dopamine D4 Receptor Gene in Stabled Horses are Related to Differences in Behavioral Response to Frustration

Animals

Shigeru Ninomiya¹  Akiko Anjiki⁴  Yudai Nishide²  Minori Mori³  Yoshitaka Deguchi³ 

[1] Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido Gifu 501-1193, Japan;Locust Research Laboratory, National Institute of Agro-biological Sciences at Ohwashi, Tsukuba, Ibaraki 305-8634, Japan; E-Mail:;Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan; E-Mails:;Department of Veterinary and Medicine Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo 183-8509, Japan; E-Mails:

关键词: animal welfare;    behaviour;    frustration;    DRD4 gene;    SNPs;    stabled horse;   

DOI  :  10.3390/ani3030663

来源: mdpi

【 摘 要 】

In this study, the association between behavioral responses to frustration and DRD4 polymorphisms was investigated in horses. Twenty one horses were observed for 4–4.5 h during feeding for several days. Horses were then genotyped for the DRD4 gene. Behavioral responses to frustration were recorded more frequently in horses without the A allele than in horses with the A allele. These results indicate that polymorphisms of DRD4 should be considered when assessing the welfare of stabled horses, by means of behavioral indicators of frustration.

In stabled horses, behavioral responses to frustration are often observed, especially around feeding time. These behavioral responses are a useful indicator of their welfare. In this study, we investigated the association between this behavioral indicator and DRD4 gene polymorphisms in stabled horses. Twenty one horses housed in two stables were used. The horses were observed for approximately 4 h around feeding over three or more days using focal-sampling and instantaneous-sampling. Horses were genotyped for the A–G substitution in the DRD4 gene. The effects of the A–G substitution (with or without the A allele in the DRD4 gene), the stables, and their interaction on the frequency of behavioral responses to frustration were analyzed using general linear models. The total time budget of behavioral responses to frustration was higher in horses without the A allele than in those with the A allele (P = 0.007). These results indicate that the A–G substitution of the DRD4 gene is related to frustration-related behavioral responses in stabled horses. Appropriate consideration should be made for the DRD4 gene polymorphism when the welfare of stabled horses is assessed, based on this behavioral indicator.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.