期刊论文详细信息
Metabolites
Electrospray Quadrupole Travelling Wave Ion Mobility Time-of-Flight Mass Spectrometry for the Detection of Plasma Metabolome Changes Caused by Xanthohumol in Obese Zucker (fa/fa) Rats
Samanthi I. Wickramasekara2  Fereshteh Zandkarimi2  Jeff Morré2  Jay Kirkwood1  LeeCole Legette1  Yuan Jiang4  Adrian F. Gombart3  Jan F. Stevens1 
[1] Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR 97331, USA; E-Mails:;Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA; E-Mails:;Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; E-Mail:;Department of Statistics, Oregon State University, Corvallis, OR 97331, USA; E-Mail:
关键词: obesity;    xanthohumol;    travelling wave ion mobility;    mass spectrometry;    lipidomics;   
DOI  :  10.3390/metabo3030701
来源: mdpi
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【 摘 要 】

This study reports on the use of traveling wave ion mobility quadrupole time-of-flight (ToF) mass spectrometry for plasma metabolomics. Plasma metabolite profiles of obese Zucker fa/fa rats were obtained after the administration of different oral doses of Xanthohumol; a hop-derived dietary supplement. Liquid chromatography coupled data independent tandem mass spectrometry (LC-MSE) and LC-ion mobility spectrometry (IMS)-MSE acquisitions were conducted in both positive and negative modes using a Synapt G2 High Definition Mass Spectrometry (HDMS) instrument. This method provides identification of metabolite classes in rat plasma using parallel alternating low energy and high energy collision spectral acquisition modes. Data sets were analyzed using pattern recognition methods. Statistically significant (p < 0.05 and fold change (FC) threshold > 1.5) features were selected to identify the up-/down-regulated metabolite classes. Ion mobility data visualized using drift scope software provided a graphical read-out of differences in metabolite classes.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.

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