Protective Effects of Alkaloid Compounds from Nelumbinis Plumula on tert-Butyl Hydroperoxide-Induced Oxidative Stress

Molecules

Yong Xie¹  Yi Zhang¹  Long-Tao Zhang¹  Shao-Xiao Zeng¹  Ze-Bin Guo¹ 

[1] College of Food Science and technology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

关键词: cytoprotection;    alkaloid;    oxidative stress;    Nelumbinis Plumula;   

DOI  :  10.3390/molecules180910285

来源: mdpi

【 摘 要 】

This study was conducted to investigate the effect of Nelumbinis Plumula total alkaloid (NPA) and its main alkaloid components on oxidative stress induced by tert-butyl hydroperoxide (t-BHP) in the human hepatocellular HepG2 cell line. According to HPLC analysis, several major alkaloid compounds such as liensinine, isoliensinine and neferine were present in NPA. The cytotoxic effects in 0.55 mM t-BHP-induced HepG2 cells were significantly inhibited by NPA and the major compound in NPA, neferine, showed the strongest activities. The protective effect of neferine against oxidative stress induced by t-BHP may be associated with decreased ROS formation, TBARS generation, LDH release and increased GSH levels, suggesting their involvement of the cytoprotective on oxidative stress. The effects were comparable with quercetin, which was used as positive control. Overall, total alkaloid and alkaloid compounds from Nelumbinis Plumula displayed a significant cytoprotective effect against oxidative stress. Further study is needed to elucidate the relationship between the chemical structures of the components in NPA and their protective effect on oxidative stress.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.