International Journal of Molecular Sciences | |
Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia | |
Umme Aminun Naher2  Radziah Othman2  Mohammad Abdul Latif3  Qurban Ali Panhwar1  Puteri Aminatulhawa Megat Amaddin1  | |
[1] Department of Land Management, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; E-Mails:;Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; E-Mails:;Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh; E-Mail: | |
关键词: diazotrophs; nitrogenase enzyme activity; indoleacetic acid; scanning electron microscopy; transmission electron microscopy; 16S rRNA; | |
DOI : 10.3390/ijms140917812 | |
来源: mdpi | |
【 摘 要 】
This study was conducted to evaluate selected biomolecular characteristics of rice root-associated diazotrophs isolated from the Tanjong Karang rice irrigation project area of Malaysia. Soil and rice plant samples were collected from seven soil series belonging to order Inceptisol (USDA soil taxonomy). A total of 38 diazotrophs were isolated using a nitrogen-free medium. The biochemical properties of the isolated bacteria, such as nitrogenase activity, indoleacetic acid (IAA) production and sugar utilization, were measured. According to a cluster analysis of Jaccard’s similarity coefficients, the genetic similarities among the isolated diazotrophs ranged from 10% to 100%. A dendogram constructed using the unweighted pair-group method with arithmetic mean (UPGMA) showed that the isolated diazotrophs clustered into 12 groups. The genomic DNA rep-PCR data were subjected to a principal component analysis, and the first four principal components (PC) accounted for 52.46% of the total variation among the 38 diazotrophs. The 10 diazotrophs that tested highly positive in the acetylene reduction assay (ARA) were identified as
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland
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