International Journal of Environmental Research and Public Health | |
Can Probiotics Improve the Environmental Microbiome and Resistome of Commercial Poultry Production? | |
Adriana A. Pedroso1  Anne L. Hurley-Bacon3  Andrea S. Zedek4  Tiffany W. Kwan1  Andrea P. O. Jordan5  Gloria Avellaneda2  Charles L. Hofacre1  Brian B. Oakley6  Stephen R. Collett1  John J. Maurer1  | |
[1] Poultry Diagnostic and Research Center/Center for Food Safety, The University of Georgia, Athens, GA 30602, USA; E-Mails:;Loehmann Animal Health International, Waterville, ME 0491, USA; E-Mail:;Merial, Athens, GA 30601, USA; E-Mail:;Zoetis Animal Health, Madison, NJ 07932, USA; E-Mail:;Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; E-Mail:;Richard B. Russell Agricultural Research Center, Agricultural Research Service, South Atlantic Area, Athens, GA 30605, USA; E-Mail: | |
关键词: antibiotic; growth promoter; microbiota; litter; probiotic; prebiotic; streptogramin; integron; | |
DOI : 10.3390/ijerph10104534 | |
来源: mdpi | |
【 摘 要 】
Food animal production systems have become more consolidated and integrated, producing large, concentrated animal populations and significant amounts of fecal waste. Increasing use of manure and litter as a more “natural” and affordable source of fertilizer may be contributing to contamination of fruits and vegetables with foodborne pathogens. In addition, human and animal manure have been identified as a significant source of antibiotic resistance genes thereby serving as a disseminator of resistance to soil and waterways. Therefore, identifying methods to remediate human and animal waste is critical in developing strategies to improve food safety and minimize the dissemination of antibiotic resistant bacteria. In this study, we sought to determine whether withdrawing antibiotic growth promoters or using alternatives to antibiotics would reduce the abundance of antibiotic resistance genes or prevalence of pathogens in poultry litter. Terminal restriction fragment length polymorphism (T-RFLP) paired with high throughput sequencing was used to evaluate the bacterial community composition of litter from broiler chickens that were treated with streptogramin growth-promoting antibiotics, probiotics, or prebiotics. The prevalence of resistance genes and pathogens was determined from sequencing results or PCR screens of litter community DNA. Streptogramin antibiotic usage did not elicit statistically significant differences in Shannon diversity indices or correlation coefficients among the flocks. However, T-RFLP revealed that there were inter-farm differences in the litter composition that was independent of antibiotic usage. The litter from all farms, regardless of antibiotic usage, contained streptogramin resistance genes (
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland.
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