Parts Per Trillion Detection of 7-Aminonitrazepam by Nano-Enhanced ELISA

International Journal of Molecular Sciences

Chifang Peng¹  Xiaohui Duan¹  Shanshan Song¹ 

[1] State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; E-Mails:

关键词: 7-aminonitrazepam;    ELISA;    gold nanoparticle;    bioconjugate;   

DOI  :  10.3390/ijms141019474

来源: mdpi

【 摘 要 】

It is challenging to detect 7-aminonitrazepam (7-ANZP) residue in animal tissues simply and sensitively by the enzyme-linked sorbent immunoassay (ELISA) method. This paper demonstrates that utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as a signal probe increases the sensitivity of a traditional ELISA for 7-ANZP by nearly 20 times. The sensitivity of this ELISA for 7-ANZP was 5.6 pg/mL in buffer, and the limit of detection (LOD) of 0.18 μg/kg for 7-ANZP in urine could be achieved after the urine samples were simply hydrolyzed and diluted by buffer. This simple and sensitive method has potential application for improving the sensitivity of ELISA methods against various small molecules.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland