Arming Technology in Yeast—Novel Strategy for Whole-cell Biocatalyst and Protein Engineering

Biomolecules

Kouichi Kuroda¹ 

[1] Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; E-Mail

关键词: cell surface engineering;    arming yeast;    whole-cell biocatalyst;    molecular display;    arming technology;    cell surface display;    protein engineering;    directed evolution;    combinatorial bioengineering;    single cell analysis;   

DOI  :  10.3390/biom3030632

来源: mdpi

【 摘 要 】

Cell surface display of proteins/peptides, in contrast to the conventional intracellular expression, has many attractive features. This arming technology is especially effective when yeasts are used as a host, because eukaryotic modifications that are often required for functional use can be added to the surface-displayed proteins/peptides. A part of various cell wall or plasma membrane proteins can be genetically fused to the proteins/peptides of interest to be displayed. This technology, leading to the generation of so-called “arming technology”, can be employed for basic and applied research purposes. In this article, we describe various strategies for the construction of arming yeasts, and outline the diverse applications of this technology to industrial processes such as biofuel and chemical productions, pollutant removal, and health-related processes, including oral vaccines. In addition, arming technology is suitable for protein engineering and directed evolution through high-throughput screening that is made possible by the feature that proteins/peptides displayed on cell surface can be directly analyzed using intact cells without concentration and purification. Actually, novel proteins/peptides with improved or developed functions have been created, and development of diagnostic/therapeutic antibodies are likely to benefit from this powerful approach.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.