Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni

Biomolecules

Venkata Sai Prakash Chaturvedula¹  Steven Chen²  Oliver Yu² 

[1] Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA;

关键词: Stevia rebaudiana;    diterpene glycoside;    isolation;    structure elucidation;    spectral data;    hydrolysis studies;   

DOI  :  10.3390/biom3040733

来源: mdpi

【 摘 要 】

From the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-(3-O-β-d-glucopyranosyl-α-l-rhamnopyranosyl)-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl) ester] (1); also known as rebaudioside O having seven sugar units has been isolated. Its structural characterization has been achieved by the extensive 1D (¹H and ¹³C), and 2D NMR (COSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside O using acid and enzymatic methods to identify aglycone and sugar residues in its structure as well as their configurations.

【 授权许可】

CC BY   
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