Analysis of Gln223Agr Polymorphism of Leptin Receptor Gene in Type II Diabetic Mellitus Subjects among Malaysians

International Journal of Molecular Sciences

Ali Etemad³  Vasudevan Ramachandran⁵  Seyyed Reza Pishva³  Farzad Heidari³  Ahmad Fazli Abdul Aziz⁴  Ahmad Khairuddin Mohamed Yusof¹  Chong Pei Pei² 

[1]Department of Cardiology, National Heart Institute, Kuala Lumpur, Selangor 50400, Malaysia

[2] E-Mail:

[3]Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

[4] E-Mail:

[5]Genetic Research Group, Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

[6] E-Mails:

[7]Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

[8] E-Mail:

[9]Institute of Gerontology, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

[10] E-Mail:

关键词: leptin receptor;    polymorphism;    type 2 diabetes mellitus;    Malaysia;    restriction enzyme;   

DOI  :  10.3390/ijms140919230

来源: mdpi

【 摘 要 】

Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Mellitus (T2DM), as well as in cardiovascular diseases (CVD). The Leptin Receptor (LEPR) gene and its polymorphisms have not been extensively studied in relation to the T2DM and its complications in various populations. In this study, we have determined the association of Gln223Agr loci of LEPR gene in three ethnic groups of Malaysia, namely: Malays, Chinese and Indians. A total of 284 T2DM subjects and 281 healthy individuals were recruited based on International Diabetes Federation (IDF) criteria. Genomic DNA was extracted from the buccal specimens of the subjects. The commercial polymerase chain reaction (PCR) method was carried out by proper restriction enzyme MSP I to both amplify and digest the Gln223Agr polymorphism. The p-value among the three studied races was 0.057, 0.011 and 0.095, respectively. The values such as age, WHR, FPG, H_bA_1C, LDL, HDL, Chol and Family History were significantly different among the subjects with Gln223Agr polymorphism of LEPR (p < 0.05).

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland