Anti-Inflammatory Activity of Iridoids and Verbascoside Isolated from Castilleja tenuiflora

Molecules

Danae Carrillo-Ocampo¹  Sugeyla Bazaldྪ-Gómez¹  Jaime R. Bonilla-Barbosa³  Rola Aburto-Amar² 

[1]Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa., Cuernavaca 62209, Morelos, Mexico

[2] E-Mails:

[3]Instituto de Farmacobiología, Universidad de la Cañada, Carretera Teotitlán - San Antonio Nanahuatipán Km 1.7 s/n., Paraje Titlacuatitla, Teotitlán de Flores Magón 68540, Oax., Mexico

[4] E-Mail:

[5]Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa., Cuernavaca 62209, Morelos, Mexico

[6] E-Mail:

关键词: Castilleja tenuiflora;    anti-inflammatory activity;    iridoids;    TPA;   

DOI  :  10.3390/molecules181012109

来源: mdpi

【 摘 要 】

Castilleja tenuiflora (Orobanchaceae) has been used in Mexican traditional medicine as a treatment for cough, dysentery, anxiety, nausea and vomiting as well as hepatic and gastrointestinal diseases. The ethanolic extract of the aerial parts of Castilleja tenuiflora was separated by silica gel column chromatography. The fractions were evaluated using the induced edema acetate 12-O-tetradecanoylphorbol (TPA) anti-inflammatory activity model. The most active fraction was subjected to medium-pressure liquid chromatography (MPLC) with UV detection at 206 and 240 nm. The following iridoids were isolated: geniposidic acid, aucubin, bartioside, 8-epi-loganin, mussaenoside, and the phenylpropanoid verbascoside. The most active iridoid was geniposidic acid, which was more active than the control (indomethacin), and the least active iridoid was mussaenoside. 8-epi-Loganin, and mussaenoside have not been previously reported to be anti-inflammatory compounds. The results of these investigations confirm the potential of Mexican plants for the production of bioactive compounds and validate the ethnomedical use of Castilleja tenuiflora-like anti-inflammatory plants.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.