Qualitative and Quantitative Analysis of Andrographis paniculata by Rapid Resolution Liquid Chromatography/Time-of-Flight Mass Spectrometry

Molecules

Yong-Xi Song²  Shi-Ping Liu²  Zhao Jin²  Jian-Fei Qin¹ 

[1] Harbin Sanlian Pharmaceutical Co., LTD, Harbin 150025, China;Department of Pharmacy, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China; E-Mails:

关键词: Andrographis paniculata;    rapid resolution liquid chromatography;    time-of-flight tandem mass;    diterpenoid lactones;   

DOI  :  10.3390/molecules181012192

来源: mdpi

【 摘 要 】

A rapid resolution liquid chromatography/time-of-flight tandem mass spectrometry (RRLC-TOF/MS) method was developed for qualitative and quantitative analysis of the major chemical constituents in Andrographis paniculata. Fifteen compounds, including flavonoids and diterpenoid lactones, were unambiguously or tentatively identified in 10 min by comparing their retention times and accurate masses with standards or literature data. The characteristic fragmentation patterns of flavonoids and diterpenoid lactones were summarized, and the structures of the unknown compounds were predicted. Andrographolide, dehydroandrographolide and neoandrographolide were further quantified as marker substances. It was found that the calibration curves for all analytes showed good linearity (R² > 0.9995) within the test ranges. The overall limits of detection (LODs) and limits of quantification (LOQs) were 0.02 μg/mL to 0.06 μg/mL and 0.06 μg/mL to 0.2 μg/mL, respectively. The relative standard deviations (RSDs) for intra- and inter-day precisions were below 3.3% and 4.2%, respectively. The mean recovery rates ranged from 96.7% to 104.5% with the relative standard deviations (RSDs) less than 2.72%. It is concluded that RRLC-TOF/MS is powerful and practical in qualitative and quantitative analysis of complex plant samples due to time savings, sensitivity, precision, accuracy and lowering solvent consumption.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.