期刊论文详细信息
International Journal of Molecular Sciences
The Key Enzyme of the Sialic Acid Metabolism Is Involved in Embryoid Body Formation and Expression of Marker Genes of Germ Layer Formation
Wenke Weidemann1  Jessica Hering1  Dorit Bennmann1  Annett Thate1 
[1] Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Hollystr.1, Halle D-06114, Germany;
关键词: sialic acid biosynthesis;    UDP-N-acetyl-2-epimerase;    N-acetylmannosamine kinase;    embryonic stem cells;    embryoid bodies;    gene expression;   
DOI  :  10.3390/ijms141020555
来源: mdpi
PDF
【 摘 要 】

The bi-functional enzyme UDP-N-acetyl-2-epimerase/N-acetylmannosamine kinase (GNE) is the key enzyme of the sialic acid biosynthesis. Sialic acids are negatively charged nine carbon amino sugars and are found on most glycoproteins and many glycolipids in terminal positions, where they are involved in a variety of biological important molecular interactions. Inactivation of the GNE by homologous recombination results in early embryonic lethality in mice. Here, we report that GNE-deficient embryonic stem cells express less differentiation markers compared to wild-type embryonic stem cells. As a result, GNE-deficient embryonic stem cells fail to form proper embryoid bodies (EB) within the first day of culture. However, when culturing these cells in the presence of sialic acids for three days, also GNE-deficient embryonic stem cells form normal EBs. In contrast, when culturing these cells in sialic acid reduced medium, GNE-deficient embryonic stem cells proliferate faster and form larger EBs without any change in the expression of markers of the germ layers.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland

【 预 览 】
附件列表
Files Size Format View
RO202003190032668ZK.pdf 490KB PDF download
  文献评价指标  
  下载次数:9次 浏览次数:12次