A Comparative Proteomic Analysis of Pinellia ternata Leaves Exposed to Heat Stress

International Journal of Molecular Sciences

Yunhao Zhu¹  Guosheng Zhu²  Qiaosheng Guo¹  Zaibiao Zhu¹  Changlin Wang¹ 

[1] Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China; E-Mails:;Institute of Morden Chinese Medical Materials, Guizhou Academy of Agricaltural Sciences, Guiyang 550006, China; E-Mail:

关键词: proteomics;    quantitative real-time PCR;    heat stress;    Pinellia ternata;   

DOI  :  10.3390/ijms141020614

来源: mdpi

【 摘 要 】

Pinellia ternata is an important traditional Chinese medicinal plant. The growth of P. ternata is sensitive to high temperatures. To gain a better understanding of heat stress responses in P. ternata, we performed a comparative proteomic analysis. P. ternata seedlings were subjected to a temperature of 38 °C and samples were collected 24 h after treatment. Increased relative ion leakage and lipid peroxidation suggested that oxidative stress was frequently generated in rice leaves exposed to high temperature. Two-dimensional electrophoresis (2-DE) was used to analyze heat-responsive proteins. More than 600 protein spots were reproducibly detected on each gel; of these spots, 20 were up-regulated, and 7 were down-regulated. A total of 24 proteins and protein species were successfully identified by MALDI-TOF/TOF MS. These proteins and protein species were found to be primarily small heat shock proteins (58%) as well as proteins involved in RNA processing (17%), photosynthesis (13%), chlorophyll biosynthetic processes (4%), protein degradation (4%) and defense (4%). Using 2-DE Western blot analysis, we confirmed the identities of the cytosolic class II small heat shock protein (sHSPs-CII) identified by MS. The expression levels of four different proteins [cytosolic class I small heat shock protein (sHSPs-CI), sHSPs-CII, mitochondrial small heat shock protein (sHSPs-MIT), glycine-rich RNA-binding protein (GRP)] were analyzed at the transcriptional level by quantitative real-time PCR. The mRNA levels of three sHSPs correlated with the corresponding protein levels. However, GRP was down-regulated at the beginning of heat stress but then increased substantially to reach a peak after 24 h of heat stress. Our study provides valuable new insight into the responses of P. ternata to heat stress.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland