Selection of a Mimotope Peptide of S-adenosyl-l-homocysteine and Its Application in Immunoassays

Molecules

Chun Wu¹ 

[1] Health Research Institute, AIST, 1-8-31 Midorigaoka, Ikeda 563-8577, Osaka, Japan

关键词: homocysteine;    competitive assay;    phage display;    monoclonal antibody;    clinical diagnostics;   

DOI  :  10.3390/molecules181013020

来源: mdpi

【 摘 要 】

A competitive immunoassay for S-adenosyl-l-homocysteine (SAH) has been used in the clinical test for homocysteine via an enzymatic conversion reaction. Since S-adenosyl-l-homocysteine is a relatively unstable compound, we have used peptide library phage display to select a new mimotope peptide that interacts with the anti-SAH antibody. By immobilizing the synthetic peptide on solid phase as a competitive surrogate for SAH, we demonstrate its utility in a competitive ELISA assay. The linear range of the assay for SAH was 0.4–6.4 µM, in good correlation to the conventional assay using an SAH-conjugated plate. Our results show that the mimotope peptide has potential to substitute for SAH in immunoassays.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.