Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions

Sensors

Thijs Vandenryt²  Andrea Pohl¹  Bart van Grinsven²  Ronald Thoelen²  Ward De Ceuninck²  Patrick Wagner² 

[1] Fraunhofer Institute for Non Destructive Testing (IZFP), Maria-Reiche-Strasse 2, 01109 Dresden, Germany; E-Mails:;Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, 3590 Diepenbeek, Belgium; E-Mails:

关键词: SPR;    EIS;    flow-cell;    lab on chip;    Protein A;    immunoglobulin;   

DOI  :  10.3390/s131114650

来源: mdpi

【 摘 要 】

In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.