A New in Vitro Anti-Tumor Polypeptide Isolated from Arca inflata

Marine Drugs

Jian Xu²  Zhiyan Chen¹  Liyan Song¹  Lili Chen²  Jianhua Zhu²  Shuangshuang Lv² 

[1] Department of Pharmacology, Jinan University, Guangzhou 510632, China; E-Mail:;Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China; E-Mails:

关键词: Arca inflata Reeve;    polypeptide;    structure analysis;    anti-tumor activity;   

DOI  :  10.3390/md11124773

来源: mdpi

【 摘 要 】

A new in vitro anti-tumor polypeptide, coded as J2-C3, was isolated from Arca inflata Reeve and purified by diethyl-aminoethanol (DEAE)-sepharose Fast Flow anion exchange and phenyl sepharose CL-4B hydrophobic chromatography. J2-C3 was identified to be a homogeneous compound by native polyacrylamide gel electrophoresis (Native-PAGE). The purity of J2-C3 was over 99% in reversed phase-high performance liquid chromatography (RP-HPLC). The molecular weight was determined as 20,538.0 Da by electrospray-ionization mass spectrometry (ESI-MS/MS). J2-C3 was rich in Glx (Gln + Glu), Lys, and Asx (Asp + Asn) according to amino acid analysis. Four partial amino acid sequences of this peptide were determined as L/ISMEDVEESR, KNGMHSI/LDVNHDGR, AMKI/LI/LNPKKGI/LVPR and AMGAHKPPKGNEL/IGHR via MALDI-TOF/TOF-MS and de novo sequencing. Secondary structural analysis by CD spectroscopy revealed that J2-C3 had the α-helix (45.2%), β-sheet (2.9%), β-turn (26.0%) and random coil (25.9%). The anti-tumor effect of J2-C3 against human tumor cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the IC₅₀ values of J2-C3 were 65.57, 93.33 and 122.95 µg/mL against A549, HT-29 and HepG2 cell lines, respectively. Therefore, J2-C3 might be developed as a potential anti-tumor agent.

【 授权许可】

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.