Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia

Molecules

Guan-Cheng Huang²  Chia-Jung Lee³  Kun-Teng Wang⁴  Bor-Chun Weng⁵  Ting-Yi Chien⁴  Sung-Hui Tseng¹ 

[1] School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan; E-Mail:;Division of Hemato-Oncology, Department of Internal Medicine, Yuan’s General Hospital, No.162 Cheng Kung 1st Road, Kaohsiung City 80249, Taiwan; E-Mail:;Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, No.155 Section 2, Linong Street, Taipei City 11221, Taiwan; E-Mail:;School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan; E-Mails:;Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No.300 Syuefu Road, Chiayi City 60004, Taiwan; E-Mail:

关键词: Hedysarum plybotrys Handel-Mazzetti (Hedysari Radix);    Fabaceae;    Astragali Radix;    anti-inflammation;    splenocyte proliferation;    daunoblastina-induced leucopenia mice;   

DOI  :  10.3390/molecules181214862

来源: mdpi

【 摘 要 】

Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is one species used of Astragali Radix. In this study, the immunomodulatory properties of H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H₂O (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE₂, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity. Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.