Hsa-Let-7g miRNA Targets Caspase-3 and Inhibits the Apoptosis Induced by ox-LDL in Endothelial Cells

International Journal of Molecular Sciences

Yefei Zhang¹  Naiyun Chen²  Jihao Zhang² 

[1] Emergency Department, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;

关键词: hsa-let-7g;    caspase-3;    apoptosis;    ox-LDL;    endothelial cells;   

DOI  :  10.3390/ijms141122708

来源: mdpi

【 摘 要 】

It has been well confirmed ox-LDL plays key roles in the development of atherosclerosis via binding to LOX-1 and inducing apoptosis in vascular endothelial cells. Recent studies have shown ox-LDL can suppress microRNA has-let-7g, which in turn inhibits the ox-LDL induced apoptosis. However, details need to be uncovered. To determine the anti-atherosclerosis effect of microRNA has-let-7g, and to evaluate the possibility of CASP3 as an anti-atherosclerotic drug target by has-let-7g, the present study determined the role of hsa-let-7g miRNA in ox-LDL induced apoptosis in the vascular endothelial cells. We found that miRNA has-let-7g was suppressed during the ox-LDL-induced apoptosis in EAhy926 endothelial cells. In addition, overexpression of has-let-7g negatively regulated apoptosis in the endothelial cells by targeting caspase-3 expression. Therefore, miRNA let-7g may play important role in endothelial apoptosis and atherosclerosis.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland