Seed Germination-Influencing Bioactive Secondary Metabolites Secreted by the Endophyte Cladosporium cladosporioides LWL5

Molecules

Muhammad Waqas²  Abdul Latif Khan²  Liaqat Ali¹  Sang-Mo Kang²  Yoon-Ha Kim² 

[1] Department of Biological Sciences and Chemistry, College of Arts & Sciences, University of Nizwa, Nizwa 33, Oman; E-Mail:;School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea; E-Mails:

关键词: natural products;    endophytes;    lettuce seed;    growth inhibition;    seed germination;    weeds control;    allelopathy;   

DOI  :  10.3390/molecules181215519

来源: mdpi

【 摘 要 】

The present study was aimed to isolate bioactive metabolites produced by a fungal endophyte from Helianthus annuus, Capsicum annuum, and Cucumis sativus and to assess their role in seed germination. Culture filtrate of the endophyte HA-3B from H. annuus was significantly inhibitory towards the germination and growth of lettuce seeds. HA-3B was identified as Cladosporium cladosporioides LWL5 through molecular techniques. Different concentrations (100, 500 and 1000 ppm) of the ethyl acetate extract obtained from the culture inhibited the lettuce seed germination. The extract was subjected to column chromatography and a bioassay-guided isolation method, which yielded compounds 1, 2 and an oily fraction. The oily fraction, subjected to fractionation and spectroscopic techniques, resulted in the identification of 31 different constituents. Compounds 1 and 2 were identified and characterized through MS and NMR spectroscopic techniques as benzoic acid. The bioassay results showed that this compound significantly inhibited the growth and germination of lettuce seeds. In conclusion, assessing the role of endophytes harboring essential crop plants can help us to develop potentially eco-friendly herbicides.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.