Effect of Exogenous Factors on Bacteriocin Production from Lactobacillus paracasei J23 by Using a Resting Cell System

International Journal of Molecular Sciences

Huaxi Yi¹  Xue Han¹  Yanyan Yang¹  Wenli Liu¹  Hui Liu¹  Yingchun Zhang¹  Kai Sun¹  Lanwei Zhang¹ 

[1] School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China; E-Mails:

关键词: lactic acid bacteria;    bacteriocin;    Lactobacillus paracasei;    resting cell;    system;    exogenous factors;   

DOI  :  10.3390/ijms141224355

来源: mdpi

【 摘 要 】

A resting cell system was developed for bacteriocin Lac-B23 production from Lactobacillus paracasei J23. The resting cell medium contained (g/L): Glucose 20, Sodium acetate 5.0, MnSO₄ 0.25 MgSO₄ 0.5, Ammoniumhydrogencitrate 1.0, KH₂PO₄ 1.0. The resting cell incubation time and temperature were 20 h and 37 °C and the effects of exogenous factors, including amino acids, glycerol, pyruvic acid, and α-ketoglutaric acid were investigated. Cys and Gly could stimulate the production of bacteriocin, while no stimulus effect was observed for Glu, Tyr and Ala. Glycerol and pyruvic acid increased bacteriocin production and the optimum concentrations were 1% and 30 g/L, respectively. Bacteriocin could act as an inducer of its own biosynthesis. These findings are of importance for the further study of bacteriocin biosynthesis regulation and for the improvement of bacteriocin production yields.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland