Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells

International Journal of Molecular Sciences

Li Qing Du²  Yan Wang²  Chang Xu²  Jia Cao²  Qin Wang²  Hui Zhao¹  Fei Yue Fan²  Bing Wang³  Takanori Katsube³  Sai Jun Fan² 

[1] Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, Tianjin 300134, China; E-Mail:;Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China; E-Mails:;National Institute of Radiological Sciences, Chiba 263-8555, Japan; E-Mails:

关键词: ANTP-smacN7 fusion protein;    apoptosis;    radiosensitivity;   

DOI  :  10.3390/ijms141224087

来源: mdpi

【 摘 要 】

The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of radiation on the expression of X-linked inhibitor of apoptosis protein (XIAP) were detected by western blotting. The radiation-sensitising effects of ANTP-SmacN7 fusion proteins were observed by a clonogenic assay. The effects of drugs and radiation on tumour cell apoptosis were determined using Annexin V/FITC double staining. Changes in caspase-8, caspase-9 and caspase-3 were detected by western blot before and after ANTP-SmacN7 inhibition of XIAP. The ANTP-SmacN7 fusion protein could enter and accumulate in cells; in vitro XIAP expression of radiation-induced tumour cells was negatively correlated with tumour radiosensitivity. The ANTP-SmacN7 fusion protein promoted tumour cell apoptosis through the activation of caspase3. ANTP-SmacN7 fusion protein may reduce tumour cell radioresistance by inducing caspase3 activation.

【 授权许可】

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