Phylogenetic Relationships between Four Salix L. Species Based on DArT Markers

International Journal of Molecular Sciences

Jerzy A. Przyborowski¹  Paweł Sulima¹  Anna Kuszewska¹  Dariusz Załuski¹ 

[1] Departament of Plant Breeding and Seed Production, University of Warmia and Mazury, Plac Łódzki 3, Olsztyn 10-724, Poland; E-Mails:

关键词: phylogenetic relationships;    genetic diversity;    species identity;    diversity arrays technology;    DArT;    Salix;    willow;   

DOI  :  10.3390/ijms141224113

来源: mdpi

【 摘 要 】

The objectives of this study were to evaluate the usefulness of DArT markers in genotypic identification of willow species and describe genetic relationships between four willow species: Salix viminalis, S. purpurea, S. alba and S. triandra. The experimental plant material comprised 53 willow genotypes of these four species, which are popularly grown in Poland. DArT markers seem to identify Salix species with a high degree of accuracy. As a result, the examined species were divided into four distinct groups which corresponded to the four analyzed species. In our study, we observed that S. triandra was very different genetically from the other species, including S. alba which is generally classified into the same subgenus of Salix. The above corroborates the findings of other authors who relied on molecular methods to reveal that the classification of S. triandra to the subgenus Salix was erroneous. The Principal Coordinate Analysis (PCoA) and the neighbor-joining dendrogram also confirmed the clear division of the studied willow genotypes into four clusters corresponding to individual species. This confirmed the usefulness of DArT markers in taxonomic analyses and identification of willow species.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland