Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium

Materials

Shinya Yamada²  Hirotaka Maeda³  Akiko Obata²  Ulrich Lohbauer⁴  Akiko Yamamoto¹ 

[1] Biometals Group, Biomaterials Unit, Nano-Bio Field, International Center for Materials Nanoarchitechtonics (MANA), National Institute for Materials Science (NIMS), 1-1, Namiki, Tsukuba 305-0044, Japan; E-Mail:;Department of Frontier Materials, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan; E-Mails:;Center for Fostering Young and Innovative Researchers, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan;Dental Materials Lab, Dental Clinic 1, University of Erlangen-Nuremberg, Glueckstrasse 11, Erlangen 91054, Germany; E-Mail:

关键词: coating;    magnesium;    poly(l-lactic acid);    vaterite (calcium carbonate);    composite;   

DOI  :  10.3390/ma6125857

来源: mdpi

【 摘 要 】

Poly(l-lactic acid)-based films which include 60 wt % of vaterite (V) or siloxane-containing vaterite (SiV) were coated on a pure magnesium substrate, denoted by PLLA/V or PLLA/SiV, respectively, to suppress early corrosion and improve its cytocompatibility. Both coating films adhered to the Mg substrate with 2.3–2.8 MPa of tensile bonding strength. Soaking test for 7 days in α-modified minimum essential medium revealed that the morphological instability of the PLLA/V film caused a higher amount of Mg²⁺ ion to be released from the coating sample. On the other hand, in the case of the coating with the PLLA/SiV film, no morphological change even after the soaking test was observed, owing to the suppression of the degradation rate. In cell culture tests, the proliferation of mouse osteoblast-like cell (MC3T3-E1) was significantly enhanced by both coatings, in comparison with the uncoated magnesium substrate. The cell morphology revealed that a few less-spread cells were observed on the PLLA/V film, while more elongated cells were done on the PLLA/SiV film. The cells on the PLLA/SiV film exhibited an extremely higher alkaline phosphatase activity after 21 days of incubation than that on the PLLA/V one. The PLLA/SiV film suppressed the early corrosion and enhanced cytocompatibility on metallic magnesium.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.