Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial

Nutrients

You Jin Kim¹  Yoon Hee Ahn¹  Yeni Lim¹  Ji Yeon Kim²  Joohee Kim³ 

[1] Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea; E-Mails:;Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul 139-743, Korea; E-Mail:;BioFood Network, Ewha Womans University, Seoul 120-160, Korea; E-Mail:

关键词: multi-micronutrient supplement;    nutritional dose;    phytochemicals;    human intervention;   

DOI  :  10.3390/nu5125218

来源: mdpi

【 摘 要 】

Reactive oxygen species are important risk factors for age-related diseases, but they also act as signaling factors for endogenous antioxidative defense. The hypothesis that a multi-micronutrient supplement with nutritional doses of antioxidant nutrients and phytochemicals (MP) may provide protection against oxidative damage and maintain the endogenous antioxidant defense capacity was assessed in subjects with a habitually low intake of fruits and vegetables. In a randomized, placebo-controlled, and parallel designed trial, 89 eligible subjects were assigned to either placebo or MP for eight weeks. Eighty subjects have completed the protocol and included for the analysis. MP treatment was superior at increasing serum folate (p < 0.0001) and resistance to DNA damage (p = 0.006, tail intensity; p = 0.030, tail moment by comet assay), and LDL oxidation (p = 0.009) compared with the placebo. Moreover, the endogenous oxidative defense capacity was not weakened after MP supplementation, as determined by the levels of glutathione peroxidase (p = 0.442), catalase (p = 0.686), and superoxide dismutase (p = 0.804). The serum folate level was negatively correlated with DNA damage (r = −0.376, p = 0.001 for tail density; r = −0.329, p = 0.003 for tail moment), but no correlation was found with LDL oxidation (r = −0.123, p = 0.275). These results suggest that MP use in healthy subjects with habitually low dietary fruit and vegetable intake may be beneficial in providing resistance to oxidative damage to DNA and LDL without suppressing the endogenous defense mechanisms.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.