Resolving Mixed Algal Species in Hyperspectral Images

Sensors

Mehrube Mehrubeoglu¹  Ming Y. Teng¹ 

[1] Hyperspectral Optical Property Instrumentation (HOPI) Laboratory, School of Engineering and Computing Sciences, Texas A&M University-Corpus Christi, 6300 Ocean Dr., Corpus Christi, TX 78412-5797, USA

关键词: hyperspectral imaging;    HSI;    hyperspectral imaging system;    HIS;    spectral response;    Beer-Lambert Law;    endmember extraction;    linear mixing model;    constrained linear unmixing;   

DOI  :  10.3390/s140100001

来源: mdpi

【 摘 要 】

We investigated a lab-based hyperspectral imaging system's response from pure (single) and mixed (two) algal cultures containing known algae types and volumetric combinations to characterize the system's performance. The spectral response to volumetric changes in single and combinations of algal mixtures with known ratios were tested. Constrained linear spectral unmixing was applied to extract the algal content of the mixtures based on abundances that produced the lowest root mean square error. Percent prediction error was computed as the difference between actual percent volumetric content and abundances at minimum RMS error. Best prediction errors were computed as 0.4%, 0.4% and 6.3% for the mixed spectra from three independent experiments. The worst prediction errors were found as 5.6%, 5.4% and 13.4% for the same order of experiments. Additionally, Beer-Lambert's law was utilized to relate transmittance to different volumes of pure algal suspensions demonstrating linear logarithmic trends for optical property measurements.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.