Heat-killed VSL#3 Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Experimental Colitis in Rats

International Journal of Molecular Sciences

Li-Xuan Sang²  Bing Chang¹  Cong Dai¹  Nan Gao²  Wei-Xin Liu¹ 

[1]Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China

[2] E-Mails:

[3]Department of Cadre Ward II, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China

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关键词: probiotic;    ulcerative colitis;    inflammatory bowel disease;    experimental colitis;    IL-6/STAT3;   

DOI  :  10.3390/ijms15010015

来源: mdpi

【 摘 要 】

To determine the effects of heat-killed VSL#3 (B. breve, B. longum and B. infantis; L. plantarum, L. bulgaricus, L. casei and L. acidophilus; S. salivarius subsp. thermophilus) therapy in the dextran sulfate sodium (DSS)-induced acute experimental colitis in rats. Acute experimental colitis was induced in rats by 5% DSS and freely drink for seven days. Beginning on Day 8, rats underwent gavage once daily for seven days with heat-killed probiotic VSL#3 (0.6 g/kg/day), colonic damage was evaluated histologically and biochemically seven days after gavage. Expression of inflammatory related mediators (STAT3, P-STAT3) and cytokines (IL-6, IL-23, TGFβ) in colonic tissue were detected. The results revealed that heat-killed and live VSL#3 have identical anti-inflammatory properties by the assessed DAI (disease activity index), colon length, histological tissue and MPO activity. Heat-killed and live VSL#3 results in reduced IL-6, IL-23, TGFβ, STAT3 and P-STAT3 expression in colonic tissue. Heat-killed and live VSL#3 have showed the similar anti-inflammatory activity by inhibiting IL-6/STAT3 pathway in the DSS-induced acute experimental colitis in rats.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland