Pharmacokinetic Comparison of Berberine in Rat Plasma after Oral Administration of Berberine Hydrochloride in Normal and Post Inflammation Irritable Bowel Syndrome Rats

International Journal of Molecular Sciences

Zipeng Gong²  Ying Chen²  Ruijie Zhang²  Yinghan Wang²  Yan Guo²  Qing Yang²  Haixian Zhang³  Yu Dong¹  Xiaogang Weng²  Shuangrong Gao² 

[1] Guang’an Men Hospital, China Academy of Chinese Medical Sciences, No.5, Beixiange Road, Xicheng District, Beijing 100053, China; E-Mail:;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing 100700, China; E-Mails:;Institute of Ethnic Medicine, Southwest University, Chengdu 610041, China; E-Mail:

关键词: berberine;    pharmacokinetic;    absorption;    irritable bowel syndrome;   

DOI  :  10.3390/ijms15010456

来源: mdpi

【 摘 要 】

In the present study, post inflammation irritable bowel syndrome (PI-IBS) rats were firstly established by intracolonic instillation of acetic acid with restraint stress. Then the pharmacokinetics of berberine in the rat plasma were compared after oral administration of berberine hydrochloride (25 mg/kg) to normal rats and PI-IBS rats. Quantification of berberine in the rat plasma was achieved by using a sensitive and rapid UPLC-MS/MS method. Plasma samples were collected at 15 different points in time and the pharmacokinetic parameters were analyzed by WinNonlin software. Compared with the normal group, area under the plasma concentration vs. time curve from zero to last sampling time (AUC_0–t) and total body clearance (CL/F) in the model group significantly increased or decreased, (2039.49 ± 492.24 vs. 2763.43 ± 203.14; 4999.34 ± 1198.79 vs. 3270.57 ± 58.32) respectively. The results indicated that the pharmacokinetic process of berberine could be altered in PI-IBS pathological conditions.

【 授权许可】

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