An Improved Neutral α-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method

Diagnostics

Frank Eertmans²  Veerle Bogaert²  Tanita Van Poecke¹ 

[1] Laboratory of Andrology, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, OVL, Belgium; E-Mail:;Department of Research and Development, FertiPro NV, Industriepark Noord 32, 8730 Beernem, WVL, Belgium; E-Mails:

关键词: epididymis;    neutral α-glucosidase;    method optimization;    assay validation;   

DOI  :  10.3390/diagnostics4010001

来源: mdpi

【 摘 要 】

Neutral α-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.