Conserved miRNAs and Their Response to Salt Stress in Wild Eggplant Solanum linnaeanum Roots

International Journal of Molecular Sciences

Yong Zhuang¹  Xiao-Hui Zhou² 

[1] Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;

关键词: salt stress;    miRNA;    Solanum linnaeanum;    high-throughput sequencing;   

DOI  :  10.3390/ijms15010839

来源: mdpi

【 摘 要 】

The Solanaceae family includes some important vegetable crops, and they often suffer from salinity stress. Some miRNAs have been identified to regulate gene expression in plant response to salt stress; however, little is known about the involvement of miRNAs in Solanaceae species. To identify salt-responsive miRNAs, high-throughput sequencing was used to sequence libraries constructed from roots of the salt tolerant species, Solanum linnaeanum, treated with and without NaCl. The sequencing identified 98 conserved miRNAs corresponding to 37 families, and some of these miRNAs and their expression were verified by quantitative real-time PCR. Under the salt stress, 11 of the miRNAs were down-regulated, and 3 of the miRNAs were up-regulated. Potential targets of the salt-responsive miRNAs were predicted to be involved in diverse cellular processes in plants. This investigation provides valuable information for functional characterization of miRNAs in S. linnaeanum, and would be useful for developing strategies for the genetic improvement of the Solanaceae crops.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland