Simultaneous Determination of Fifteen Constituents of Jitai Tablet Using Ultra High-Performance Liquid Chromatography Coupled with Triple Quadrupole Electrospray Tandem Mass Spectrometry

Molecules

Shuping Wang³  Peng Fu¹  Lei Liu²  Lingling Wang⁵  Chengcheng Peng⁴  Weidong Zhang³ 

[1] Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; E-Mail:;College of Pharmacy, Nankai University, Tianjin 300071, China; E-Mail:;School of Pharmacy, Second Military Medical University, Shanghai 200433, China; E-Mail:;School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; E-Mail:;National Engineering Research Center for TCM, Shanghai 201203, China; E-Mail:

关键词: Jitai tablet;    UHPLC-ESI-MS/MS;    quality evaluation;    TCMP;   

DOI  :  10.3390/molecules19021635

来源: mdpi

【 摘 要 】

An ultra-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was developed for simultaneous determination of fifteen constituents in Jitai tablet (JTT), a complex Traditional Chinese Medicine prescription (TCMP) used in treating opiate addiction. Benefitting from a small particle size (1.8 µm) C18 column, accelerated analysis with satisfactory resolution, sensitivity and selectivity were achieved in a single run within 7 min with linear gradient elution of acetonitrile-0.1% (v/v) formic acid in water. The analytical signal was obtained by multiple reaction monitoring transitions via electrospray ionization source operating in both positive and negative ionization mode. The approach was validated for linearity, sensitivity, precision, repeatability, stability and recovery. All analytes showed good linearity over a wide concentration range (r > 0.99). The method limits ranged from 0.03 ng/mL to 19.35 ng/mL which are sensitive enough for quality control studies. The developed method was successfully applied to the simultaneous determination of fifteen constituents in JTT. In conclusion, our experimental results demonstrate that UHPLC-ESI-MS/MS is a useful approach for the overall quality assessment of complex TCMPs.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.