Deoxynivalenol and Oxidative Stress Indicators in Winter Wheat Inoculated with Fusarium graminearum

Toxins

Agnieszka Waśkiewicz¹  Iwona Morkunas⁴  Waldemar Bednarski²  Van Chung Mai⁴  Magda Formela⁴  Monika Beszterda¹  Halina Wiśniewska³ 

[1] Department of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, Poznań 60-625, Poland; E-Mails:;Institute of Molecular Physics, Polish Academy of Sciences, Smoluchowskiego 17, Poznań 60-179, Poland; E-Mail:;Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, Poznań 60-479, Poland; E-Mail:;Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, Poznań 60-637, Poland; E-Mails:

关键词: deoxynivalenol;    semiquinone radicals;    Fusarium graminearum;    oxidative stress;    winter wheat;    zearalenone;    ergosterol;   

DOI  :  10.3390/toxins6020575

来源: mdpi

【 摘 要 】

This study comprises analyses of contents of mycotoxins, such as deoxynivalenol and zearalenone, as well as the level of oxidative stress in ears of a susceptible wheat cultivar Hanseat and cv. Arina, resistant to a pathogenic fungus Fusarium graminearum. Starting from 48 h after inoculation, a marked increase was observed in the contents of these mycotoxins in ears of wheat; however, the greatest accumulation was recorded in the late period after inoculation, i.e., during development of disease. Up to 120 h after inoculation, in ears of both wheat cultivars, the level of deoxynivalenol was higher than that of zearalenone. The susceptible cultivar was characterized by a much greater accumulation of deoxynivalenol than the resistant cultivar. At the same time, in this cultivar, in the time from 0 to 72 h after inoculation, a marked post-infection increase was observed in the generation of the superoxide radical (O₂^•−). Additionally, its level, at all the time points after inoculation, was higher than in the control. In wheat cv. Arina, a markedly higher level of O₂^•− generation in relation to the control was found up to two hours after inoculation and, next, at a later time after inoculation. In turn, the level of semiquinone radicals detected by electron paramagnetic resonance (EPR) increased at later culture times, both in cv. Hanseat and Arina; however, in infested ears of wheat, it was generally lower than in the control. Analysis of disease symptoms revealed the presence of more extensive lesions in ears of a susceptible wheat cv. Hanseat than resistant cv. Arina. Additionally, ergosterol level as a fungal growth indicator was higher in ears of susceptible wheat than in the resistant cultivar.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.