Optimization of High-Pressure Ultrasonic-Assisted Simultaneous Extraction of Six Major Constituents from Ligusticum chuanxiong Rhizome using Response Surface Methodology

Molecules

Jin-Liang Liu¹  Shun-Lin Zheng¹  Qiao-Jia Fan²  Ji-Chao Yuan¹  Shi-Min Yang¹ 

[1] College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China; E-Mails:;College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China; E-Mail:

关键词: Ligusticum chuanxiong;    major constituents;    response surface methodology;    optimization;    high-pressure ultrasonic-assisted extraction;   

DOI  :  10.3390/molecules19021887

来源: mdpi

【 摘 要 】

High-pressure ultrasound-assisted extraction technology was applied to extract ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, ligustilide and levistolide A from Ligusticum chuanxiong rhizomes. Seven independent variables, including solvent type, pressure, particle size, liquid-to-solid ratio, extraction temperature, ultrasound power, and extraction time were examined. Response Surface Methodology (RSM) using a Central Composite Design (CCD) was employed to optimize the experimental conditions (extraction temperature, ultrasonic power, and extraction time) on the basis of the results of single factor tests for the extraction of these six major components in L. chuanxiong rhizomes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and were also examined using appropriate statistical methods. The best extraction conditions were as follows: extraction solvent: 40% ethanol; pressure: 10 MPa; particle size: 80 mesh; liquid-to-solid ratio: 100:1; extraction temperature: 70 °C; ultrasonic power, 180 W; and extraction time, 74 min.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.