The Effect of Exogenous Zinc Concentration on the Responsiveness of MC3T3-E1 Pre-Osteoblasts to Surface Microtopography: Part II (Differentiation)

Materials

Kathryn Dorst¹  Derek Rammelkamp¹  Michael Hadjiargyrou² 

[1] Department of Materials Science and Engineering, Stony Brook University, Stony Brook, NY 11794-2275, USA; E-Mails:;Department of Life Sciences, New York Institute of Technology, Old Westbury, NY 11568-8000, USA; E-Mail:

关键词: osteoblast;    zinc;    micropatterns;    transforming growth factor-beta 1;    metalloproteinase-2;   

DOI  :  10.3390/ma7021097

来源: mdpi

【 摘 要 】

Osseointegration of bone implants is a vital part of the recovery process. Numerous studies have shown that micropatterned geometries can promote cell-substrate associations and strengthen the bond between tissue and the implanted material. As demonstrated previously, exogenous zinc levels can influence the responsiveness of pre-osteoblasts to micropatterns and modify their migratory behavior. In this study, we sought to determine the effect of exogenous zinc on differentiation of osteoblasts cultured on micropatterned vs. planar substrates. Levels of activated metalloproteinase-2 (MMP-2) and transforming growth factor-beta 1 (TGF-β1), as well as early stage differentiation marker alkaline phosphatase, were altered with the addition of zinc. These results suggest that exogenous zinc concentration and micropatterning may interdependently modulate osteoblast differentiation.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland