Towards a Tissue-Engineered Ligament: Design and Preliminary Evaluation of a Dedicated Multi-Chamber Tension-Torsion Bioreactor

Processes

Cຝric P. Laurent²  Cຝryck Vaquette⁶  Céline Martin¹  Emmanuel Guedon¹  Xiude Wu⁵  Alain Delconte²  Dominique Dumas³  Sstien Hupont⁴  Natalia De Isla³  Rachid Rahouadj² 

[1] CNRS, LRGP, UMR 7274, Université de Lorraine, 2 Avenue de la Forêt de Haye, 54504 Vandeuvre-lès-Nancy, France; E-Mails:;CNRS, LEMTA, UMR 7563, Université de Lorraine, 2 Avenue de la Forêt de Haye, 54504 Vandeuvre-lès-Nancy, France; E-Mails:;CNRS, IMoPA, UMR 7365, Biopôle, Université de Lorraine, 9 Avenue de la Forêt de Haye, 54504 Vandeuvre-lès-Nancy, France; E-Mails:;PTIBC-IBISA, CNRS, FR3209, Biopôle, Université de Lorraine, 9 Avenue de la Forêt de Haye, 54504 Vandeuvre-lès-Nancy, France; E-Mail:;Department of Electromechanical Engineering, School of Mechanical Engineering, Yangtze University, 1 Xueyuan Road, Jingzhou 434023, China; E-Mail:;Institute of Health and Biomedical Innovation, Queensland University of Technology, Cnr Blamey Street & Musk Avenue, Kelvin Grove Brisbane, Queensland 4059, Australia; E-Mail:

关键词: bioreactor;    braided scaffold;    stem cells;    ligament tissue engineering;   

DOI  :  10.3390/pr2010167

来源: mdpi

【 摘 要 】

Tissue engineering may constitute a promising alternative to current strategies in ligament repair, providing that suitable scaffolds and culture conditions are proposed. The objective of the present contribution is to present the design and instrumentation of a novel multi-chamber tension-torsion bioreactor dedicated to ligament tissue engineering. A preliminary biological evaluation of a new braided scaffold within this bioreactor under dynamic loading is reported, starting with the development of a dedicated seeding protocol validated from static cultures. The results of these preliminary biological characterizations confirm that the present combination of scaffold, seeding protocol and bioreactor may enable us to head towards a suitable ligament tissue-engineered construct.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.