Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine

Forests

Tania Quesada³  Marcio F.R. Resende²  Patricio Muñoz⁵  Jill L. Wegrzyn⁶  David B. Neale¹  Matias Kirst³  Gary F. Peter³  Salvador A. Gezan³  C. Dana Nelson⁴ 

[1] Department of Plant Sciences, University of California at Davis, Davis, CA 95616, USA; E-Mail:;Genetics and Genomics Graduate Program, University of Florida, Gainesville, FL 32611, USA; E-Mail:;School of Forest Resources and Conservation, University of Florida, Gainesville, FL 32611, USA; E-Mails:;USDA Forest Service, Southern Research Station, Southern Institute of Forest Genetics, Saucier, MS 39574, USA; E-Mail:;Agronomy Department, University of Florida, Gainesville, FL 32611, USA; E-Mail:;Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, 60269 USA; E-Mail:

关键词: Pinus taeda;    major gene resistance;    gene-for-gene interaction;    single nucleotide polymorphism (SNP);    BayesCπ;    Bayesian Association with Missing Data (BAMD);    logarithm of the odds (LOD);   

DOI  :  10.3390/f5020347

来源: mdpi

【 摘 要 】

Fusiform rust resistance can involve gene-for-gene interactions where resistance (Fr) genes in the host interact with corresponding avirulence genes in the pathogen, Cronartium quercuum f.sp. fusiforme (Cqf). Here, we identify trees with Fr genes in a loblolly pine population derived from a complex mating design challenged with two Cqf inocula (one gall and 10 gall mixtures). We used single nucleotide polymorphism (SNP) genotypes at sufficient density to ensure linkage between segregating markers and Fr genes identifying SNPs that explained high proportions of variance in disease incidence using BayesCπ, that also were significant using Bayesian Association with Missing Data (BAMD) software. Two SNPs mapped near Fr1 and generated significant LOD scores in single marker regression analyses for Fr1/fr1 parent 17 as well as four other parents. One SNP mapped near Fr8 and was significant for parent 28. Two SNPs mapped to linkage groups not previously shown to contain Fr genes and were significant for three parents. Parent 2 showed evidence of Fr gene stacking. Our results suggest that it is feasible to identify trees segregating for Fr genes, and to map Fr genes, based on parental analysis of SNPs that cosegregate with disease incidence in designed resistance screening trials.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.