Preparation and In Vitro Evaluation of Glycyrrhetinic Acid-Modified Curcumin-Loaded Nanostructured Lipid Carriers

Molecules

Yang Chu¹  Dan Li³  Yi-Fan Luo¹  Xiao-Jin He² 

[1] Department of Pharmacy, The First Affiliated Hospital of China Medical University, 155 Nanjing Street, Shenyang 110001, China;School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Street, Shenyang 110016, China;Department of Pharmacy, Central Hospital Attached to Shenyang Medical College, No. 5 the 7th south-west Road, TieXi district, Shenyang 110024, China

关键词: curcumin;    glycyrrhetinic acid;    nanostructured lipid carriers;    anti-HepG₂;   

DOI  :  10.3390/molecules19022445

来源: mdpi

【 摘 要 】

Curcumin, a phenolic antioxidant compound derived from the rhizome of the turmeric plant Curcuma longa, has proven to be a modulator of intracellular signaling pathways that control cancer cell growth, inflammation, invasion and apoptosis, revealing its anticancer potential. In this study, a Glycyrrhetinic Acid-Modified Curcumin-Loaded Nanostructured Lipid Carrier (Cur-GA-PEG-NLC) was prepared by the film ultrasound method to improve the tumor-targeting ability. The drug content was detected by an UV spectrophotometry method. The encapsulation efficiency of curcumin in the nanostructured lipid carriers (NLCs) was determined using a mini-column centrifugation method. The encapsulation efficiency for various Cur-GA-PEG-NLC was within the range of 90.06%–95.31% and particle size was between 123.1 nm and 132.7 nm. An in vitro MTT assay showed that Cur-GA10%-PEG-NLC had significantly high cellular uptake and cytotoxicity against HepG2 cells compared with other groups.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.