Uptake of Eudragit Retard L (Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats

Materials

Mosaad A. Abdel-Wahhab⁴  Khaled G. Abdel-Wahhab¹  Fathia A. Mannaa¹  Nabila S. Hassan²  Ramia Safar³  Roudayna Diab³  Bernard Foliguet³  Luc Ferrari³ 

[1] Medical Physiology Department, National Research Center, Dokki, Cairo 12311, Egypt; E-Mails:;Pathology Department, National Research Center, Dokki, Cairo 12311, Egypt; E-Mail:;Faculty of Pharmacy, EA 3452 CITHEFOR (Cibles thérapeutiques, formulation et expertise préclinique du médicament), Lorraine University, Nancy Cedex 54001, France; E-Mails:;Food Toxicology & Contaminants Department, National Research Center, Dokki, Cairo 12311, Egypt

关键词: Eudragit;    nanoparticles;    nanotoxicity;    hematology;    erythrocytes damage;    oxidative stress;   

DOI  :  10.3390/ma7031555

来源: mdpi

【 摘 要 】

The aim of this study was to prepare Eudragit Retard L (Eudragit RL) nanoparticles (ENPs) and to determine their properties, their uptake by the human THP-1 cell line in vitro and their effect on the hematological parameters and erythrocyte damage in rats. ENPs showed an average size of 329.0 ± 18.5 nm, a positive zeta potential value of +57.5 ± 5.47 mV and nearly spherical shape with a smooth surface. THP-1 cell lines could phagocyte ENPs after 2 h of incubation. In the in vivo study, male Sprague-Dawley rats were exposed orally or intraperitoneally (IP) with a single dose of ENP (50 mg/kg body weight). Blood samples were collected after 4 h, 48 h, one week and three weeks for hematological and erythrocytes analysis. ENPs induced significant hematological disturbances in platelets, red blood cell (RBC) total and differential counts of white blood cells (WBCs) after 4 h, 48 h and one week. ENP increased met-Hb and Co-Hb derivatives and decreased met-Hb reductase activity. These parameters were comparable to the control after three weeks when administrated orally. It could be concluded that the route of administration has a major effect on the induction of hematological disturbances and should be considered when ENPs are applied for drug delivery systems.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland