Nerve Growth Factor from Cobra Venom Inhibits the Growth of Ehrlich Tumor in Mice

Toxins

Alexey V. Osipov²  Tatiana I. Terpinskaya¹  Elena V. Kryukova²  Vladimir S. Ulaschik¹  Lubov V. Paulovets¹  Elena A. Petrova¹  Ekaterina V. Blagun¹  Vladislav G. Starkov² 

[1] Institute of Physiology, National Academy of Sciences of Belarus, ul. Akademicheskaya, 28, Minsk 220072, Belarus; E-Mails:;Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia; E-Mails:

关键词: Ehrlich carcinoma;    K252a;    nerve growth factor;    TrkA receptor;    MCF-7 cell line;   

DOI  :  10.3390/toxins6030784

来源: mdpi

【 摘 要 】

The effects of nerve growth factor (NGF) from cobra venom (cvNGF) on growth of Ehrlich ascites carcinoma (EAC) cells inoculated subcutaneously in mice have been studied. The carcinoma growth slows down, but does not stop, during a course of cvNGF injections and restores after the course has been discontinued. The maximal anti-tumor effect has been observed at a dose of 8 nmoles cvNGF/kg body weight. cvNGF does not impact on lifespan of mice with grafted EAC cells. K252a, a tyrosine kinase inhibitor, attenuates the anti-tumor effect of cvNGF indicating the involvement of TrkA receptors in the process. cvNGF has induced also increase in body weight of the experimental animals. In overall, cvNGF shows the anti-tumor and weight-increasing effects which are opposite to those described for mammalian NGF (mNGF). However in experiments on breast cancer cell line MCF-7 cvNGF showed the same proliferative effects as mNGF and had no cytotoxic action on tumor cells in vitro. These data suggest that cvNGF slows down EAC growth via an indirect mechanism in which TrkA receptors are involved.

【 授权许可】

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